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KMID : 0895820110210020031
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Journal of Oriental Rehabilitation Medicine
2011 Volume.21 No. 2 p.31 ~ p.48
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Anti-obesity Effects of Wolbi-tang( ) on the Obese-mice Induced by High-fat Diet
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Park Ji-Hyun
Hong Seo-Young
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Abstract
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Objectives: In order to investigate the anti-obesity effects of Wolbi-tang(here in after referred to WBT) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high-fat diet.
Methods: C57BL/6 mice were divided into 5 groups(normal, only high-fat diet, high-fat diet with Reductil, high-fat diet with WBT 400, 200 mg/kg extract) and fed for 5 weeks. And observed body weight change, total cholesterol, low density lipoprotein cholesterol(LDL-cholesterol), high density lipoprotein cholesterol (HDL-cholesterol), triglyceride, glucose, leptin change, alanine transaminase(ALT), aspartate transaminase(AST), serum creatinine, the expression of β3-adrenergic receptor(β3AR), leptin, uncoupling protein(UCP2) gene in 3T3-L1 adipocyte, 3T3-L1 adipocyte proliferation, histological analysis of adipose tissue and liver tissue.
Results: 1. Refer to cell cytotoxicity, viability of human fibroblast cells(hFCs) showed not significant changes. 2. The amount of ALT, AST was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. The amount of creatinine showed not significant changes. 3. Body weight was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. 4. The amount of total cholesterol and triglyceride was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. LDL-cholesterol was decreased and HDL-cholesterol was increased significantly in WBT 400 mg/kg group. 5. The amount of glucose was decreased significantly in WBT 400 mg/kg group. 6. The amount of serum leptin was decreased significantly in WBT 400 mg/kg, 200 mg/kg groups. 7. The revelation of β3AR in 3T3-L1 adipocyte was increased significantly in WBT 100 μg/ml, 50 μg/ml groups. The revelation of leptin was decreased significantly in WBT 100 μg/ml, 50 μg/ml groups. The revelation of UCP2 was decreased significantly in WBT 100 μg/ml group. 8. 3T3-L1 adipocyte proliferation was decreased significantly in WBT 100 μg/ml, 50 μg/ml groups. The size of adipocyte was decreased relative to the control group in WBT 400 mg/kg group. 9. The adipose vacuoles in liver tissue was decreased relative to the control group.
Conclusions: These results suggested that WBT has inhibitory effects of obesity. WBT might be applicated on treatment of obesity and metabolic syndrome. Further studies analysing its effects were needed.
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KEYWORD
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Wolbi-tang, WBT, Obese inhibitory, Obese gene, Adipocyte, Liver tissue
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